Microlife: Spring puddle in field

Jun 14-16, 2014

This was from a broad shallow puddle in a grassy field, taken a day after rain. The water was filmy and packed full of old brown strands, extending into a circle caked all around the rim, though with some longer green tufts growing through. The sample included some dead grass and other loose debris from near the edge.

Puddle

The material was full of thin greenish filaments, especially toward the bottom, mainly cyanobacteria though various green algae were also present. Small Nostoc balls were also reasonably widespread, and in places there were countless free-swimming bacteria, most curved and apparently divided in four.

Flagellates were common throughout including green euglenids and colonies like Pandorina and Synura. There were also widespread rotifers, notably Lecane and Lepadella, as well as some amoebae or shells like euglyphids and Arcella. Larger crustaceans included a variety of water fleas as well as copepods.

↬ Thanks to protistologist Dr. Ferry Siemensma, author of Microworld, for confirming the identification of Clathrulina elegans shells.

Kingdom Bacteria
…Phylum Cyanobacteria – blue-green algae

Cyanobacteria
Cyanobacteria - about 5 µm wide
Cyanobacteria, Arcella
Cyanobacteria, Arcella - about 25-40, 45 µm
Nostocaceae
Nostocaceae - about 4-5 µm wide
Nostoc
Nostoc - sphere about 55 µm
Nostoc
Nostoc - spheres about 50, 75 µm
Nostoc
Nostoc - sphere about 125 µm

Kingdom Plantae
…Phylum Chlorophyta – green algae

Chlorophyte
Chlorophyta - about 6-19 µm wide
Chlorophyte
Chlorophyta - about 6-24 µm wide
Chaetophora
Chaetophora - filaments up to 7 µm wide
Gonium
Gonium - colony about 35 µm
Pandorina
Pandorina - colony about 22 µm
Pandorina
Pandorina - colony about 35 µm
Volvocaceae
Volvocaceae - envelope about 70 µm
Volvocaceae
Volvocaceae - envelope about 95 µm
Zygnema
Zygnema - about 27 µm wide
Spirogyra
Spirogyra - about 12 µm wide
Closterium
Closterium - about 115 µm
Cosmarium
Cosmarium - about 27 µm

Kingdom Chromista
…Phylum Cryptista

Cryptomonas
Cryptomonas - about 40 µm

…Phylum Cercozoa

Clathrulina elegans shell
Clathrulina shell - about 80 µm
Euglyphid
Euglyphida - about 60 µm
Euglyphid
Euglyphida - about 80 µm

…Phylum Ciliophora – ciliates

Climacostomum
Climacostomum - about 165 µm
Stentor
Stentor - about 255 µm
Ciliate
Ciliophora - about 45 µm
Halteria
Halteria - about 22 µm
Spirotrichea
Spirotrichea - about 45 µm
Ciliate dividing
Ciliophora, dividing - halves about 110-115 µm
Dileptid
Dileptida - to about 55 µm wide
Ciliate
Ciliophora - about 60 µm
Frontonia
Frontonia - about 85 µm
Frontonia, dividing
Frontonia, dividing - halves about 60 µm
Ciliate
Ciliophora - about 70 µm
Vorticella
Vorticella - body about 35 µm
Vorticellid
Vorticellidae - body about 55 µm
Vorticellid
Vorticellidae - body about 65 µm
Vorticellid
Vorticellidae - body about 75 µm

…Phylum Miozoa

Dinoflagellate
Peridinea - about 50 µm
Dinoflagellate
Peridinea - about 65 µm

…Phylum Ochrophyta – heterokont algae

Synura
Synura - colonies about 35, 95 µm
Synura
Chrysophyceae - colony about 65 µm
Mallomonas
Mallomonas - about 70 µm
Anthophysa
Anthophysa - clusters about 24-28 µm
Dinobryon
Dinobryon - cells about 17-20 µm
Diatoms
Diatomeae - about 40, 60 µm
Diatom
Diatomeae - about 30-40, 60, 80 µm
Diatom
Diatomeae - about 110 µm

Kingdom Protozoa
…Phylum Euglenozoa

Euglenid
Phacus - about 45 µm
Euglenid
Euglenida - about 40 µm
Euglenid
Euglenida - about 85 µm
Euglenids
Euglenida - about 95, 115 µm
Trachelomonas
Trachelomonas - about 28 µm
Trachelomonas
Trachelomonas - about 30 µm
Trachelomonas lorica?
Trachelomonas lorica? - about 35 µm

…Phylum Amoebozoa

Tubulinea
Tubulinea - about 75 µm
Arcella, Cyanobacteria
Arcella, Cyanobacteria - about 45, 25-40 µm
Arcella
Arcella - shell about 75 µm

Kingdom Animalia
…Phylum Gnathifera – rotifers & allies

Rotaria
Rotaria - about 325 µm
Lepadella
Lepadella - about 130 µm
Lepadella
Lepadella - about 175 µm
Ploima
Ploima - about 175 µm
Ploima
Ploima - about 115 µm
Ploima
Ploima - about 95 µm
Ploima
Ploima - about 470 µm
Euchlanis
Euchlanis - about 420 µm
Lecane
Lecane - body about 90 µm
Lecane
Lecane - body about 140 µm
Lophocharis
Lophocharis - body about 145 µm

…Phylum Gastrotricha

Chaetonotus
Chaetonotus - about 115 µm

…Phylum Annelida – segmented worms

Naidid
Naididae - about 3.6 mm

…Phylum Nematoda – roundworms

Roundworm
Nematoda - about 240 µm
Roundworm
Nematoda - about 425 µm

…Phylum Arthropoda – insects, crustaceans & allies

Cyclopoid
Cyclopoida - body about 355 µm
Copepod larva
Copepoda, larva - body about 85 µm
Copepod larva
Copepoda, larva - body about 140 µm
Scapholeberis
Scapholeberis - body about 570 µm
Scapholeberis
Scapholeberis - bodies about 685, 890 µm
Simocephalus
Simocephalus - body about 700 µm
Simocephalus
Simocephalus - about 1.6 mm
Ceriodaphnia
Ceriodaphnia - body about 730 µm
Daphnia
Daphnia - body about 1.5 mm
Daphnia
Daphnia - about 2.2 mm
Mide larva
Chironomidae, larva - about 820 µm
Mide larva
Chironomidae, larva - about 2.1 mm
Mide larva
Chironomidae, larva - about 5.8 mm
Diving beetle larva
Dytiscidae, larva - body about 3.5 mm

Uncertain objects

Unknown
Non-moving branched object - about 3-4 µm wide
Unknown
Non-moving rod, chain - about 20, 40 µm
Unknown
Motile bacterial chains - about 4-9 µm
Unknown
Algae on copepod moult - about 9-14 µm
Unknown
Flagellates, Diatomeae - cells about 7, 20, 40 µm
Unknown
Green flagellate - about 16 µm
Unknown
Green flagellate, Diatomeae - about 24, 40, 60 µm
Unknown
Non-moving algae - cells about 10-11 µm, 19 µm
Unknown
Dark heliozoan-like - body about 50 µm
Unknown
Very slow amoeba, Phacus - bodies about 20, 45 µm
Unknown
Small protozoan - about 26 µm
Unknown
Gliding flagellate - about 24 µm
Unknown
Layered granule - about 100 µm